Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Journal article (2020)

Authors

J.N.A. Vink BN/Stan Brouns Lab - AS , Kavli institute of nanoscience Delft
Koen J.A. Martens Wageningen University & Research
Marnix Vlot Wageningen University & Research
R. McKenzie Kavli institute of nanoscience Delft, BN/Stan Brouns Lab - AS
C. Almendros Romero BN/Stan Brouns Lab - AS , Kavli institute of nanoscience Delft
B.A. Estrada Bonilla BN/Stan Brouns Lab - AS , Kavli institute of nanoscience Delft
Daan J.W. Brocken Gorlaeus Laboratories
Johannes Hohlbein Wageningen University & Research
S.J.J. Brouns Kavli institute of nanoscience Delft, BN/Stan Brouns Lab - AS
Research Group
BN/Stan Brouns Lab (AS) (TU Delft)
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Published Date

2020

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Stan Brouns Lab

Abstract

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

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