A Novel Unspecific Peroxygenase from Galatian marginata for Biocatalytic Oxyfunctionalization Reactions

Journal article (2022)

Authors

Yunjian Ma Macau University of Science and Technology, South China University of Technology
Hongjing Liang South China University of Technology
Zexin Zhao Hubei University of Technology
Bin Wu South China University of Technology
Dongming Lan South China University of Technology
F. Hollmann BT/Biocatalysis - AS
Yonghua Wang South China University of Technology, Guangdong Youmei Institute of Inteligent Bio-manufacturing Co, Guangdong
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2022 Yunjian Ma, Hongjing Liang, Zexin Zhao, Bin Wu, Dongming Lan, F. Hollmann, Yonghua Wang
DOI: https://doi.org/10.1016/j.mcat.2022.112707
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Published Date

2022

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Unspecific peroxygenases (UPOs, EC 1.11.2.1) are promising oxyfunctionalization catalysts because of their unique stereoselectivity. However, so far only a few UPOs have been reported. In this study, gene mining was used to identify a gene from Galerina marginata that coded for a novel UPO (GmaUPO). GmaUPO was expressed in Pichia pastoris X-33 by scale-up fermentation (the UPO activity of the culture supernatant was 118 U/L). GmaUPO exhibited a molecular weight of 40 kDa and exhibited highest activity at 35°C and pH 9, respectively. Furthermore, GmaUPO was demonstrated to catalyze the epoxidation, sulfoxidation, and hydroxylation of common substrates, particularly fatty acids such as tridecanoic acid. The molecular basis for GmaUPO regioselectivity for fatty acid hydroxylation was explored by molecular modelling. The regioselectivity was mostly governed by the architecture of the enzyme's active site.