- document
-
Kim, B. (author), Ha, M. (author), Loeff, L. (author), Chang, H. (author), Simanshu, D.K. (author), Li, S. (author), Fareh, M. (author), Patel, D.J. (author), Joo, C. (author), Kim, V.N. (author)Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the absence of Lin28. We find that the...journal article 2015
- document
-
Kim, T.J. (author), Joo, C. (author), Seong, J. (author), Vafabakhsh, R. (author), Botvinick, E.L. (author), Berns, M.W. (author), Palmer, A.E. (author), Wang, N. (author), Ha, T. (author), Jakobsson, E. (author), Sun, J. (author), Wang, Y. (author)It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca2+ signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca2+, ER Ca2+ release is the source of intracellular Ca2+ oscillations induced...journal article 2015
- document
-
Kim, S.H. (author), Park, J. (author), Joo, C. (author), Kim, D. (author), Ha, T. (author)RecA proteins form a long stable filament on a single-stranded DNA and catalyze strand exchange reaction. The stability of RecA filament changes dramatically with pH, yet its detailed mechanism is not known. Here, using a single molecule assay, we determined the binding and dissociation rates of RecA monomers at the filament ends at various pH....journal article 2015
- document
-
Kim, SH (author), Joo, C. (author), Seong, A (author), Vafabakshsh, A (author), Botvinick, A (author), Berns, A (author), Palmer, TA (author), Wang, Z. (author), Ha, Seungkyu (author), Jakobsson, ME (author), Sun, M (author)It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca2+ signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca2+, ER Ca2+ release is the source of intracellular Ca2+ oscillations induced...journal article 2015
- document
-
Kim, S.H. (author), Joo, C. (author), Ha, T. (author), Kim, D. (author)A is a DNA-dependent ATPase and mediates homologous recombination by first forming a filament on a single-stranded (ss) DNA. RecA binds preferentially to TGG repeat sequence, which resembles the recombination hot spot Chi (5'-GCTGGTGG-3') and is the most frequent pattern (GTG) of the codon usage in Escherichia coli. Because of the highly dynamic...journal article 2013