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Li, Huanhuan (author), Younes, S.H.H. (author), Chen, Shaohang (author), Duan, Peigao (author), Cui, Chengsen (author), Wever, Ron (author), Zhang, Wuyuan (author), Hollmann, F. (author)In this contribution, we report chemoenzymatic bromodecarboxylation (Hunsdiecker-type) of α,ß-unsaturated carboxylic acids. The extraordinarily robust chloroperoxidase from Curvularia inaequalis (CiVCPO) generated hypobromite from H2O2 and bromide, which then spontaneously reacted with a broad range of unsaturated carboxylic acids and yielded...journal article 2022
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- Han, Jeong Woo (author), Hollmann, F. (author), Luque, Rafael (author), Song, In Kyu (author), Talarico, Giovanni (author), Tatsumi, Takashi (author), Yan, Ning (author) journal article 2022
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Kim, Jinhyun (author), Um, Yunna (author), Han, Seunghyun (author), Hilberath, T. (author), Kim, Yong Hwan (author), Hollmann, F. (author), Park, Chan Beum (author)The pulp and paper manufacturers generate approximately 50 million metric tons of lignin per annum, most of which has been abandoned or incinerated because of lignin's recalcitrant nature. Here, we report bias-free photoelectrochemical (PEC) oxidation of lignin coupled with asymmetric hydrogenation of C=C bonds. The PEC platform consists of a...journal article 2022
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Benincá, Luiza A.D. (author), França, Alexandre S. (author), Brêda, Gabriela C. (author), Leão, Raquel A.C. (author), Almeida, Rodrigo V. (author), Hollmann, F. (author), de Souza, Rodrigo O.M.A. (author)The fatty acid photodecarboxylase from Chlorella variabilis NC64A (CvFAP) promotes the elimination of CO<sub>2</sub> from fatty acids (C<sub>n</sub>) producing the corresponding hydrocarbon (<sub>Cn-1</sub>). Therefore, this enzyme is of great biotechnological interest since it can be used in alternative biofuel production routes matching the...journal article 2022
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Yang, Liu (author), Ma, Yunjian (author), Chen, Yebao (author), Hollmann, F. (author), Wang, Yonghua (author)Phthalic acid esters (PAEs) are widely used as plastic additives to increase the flexibility and durability of plastics. Constantly leaching out from plastics, PAEs are ubiquitously found in the environment. As PAEs exhibit biological activities such as being endocrine disruptive, the quest for efficient degradation strategies continues. Here...journal article 2022
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Chanquia, Santiago Nahuel (author), Benfeldt, Frederik Vig (author), Petrovai, Noémi (author), Santner, Paul (author), Hollmann, F. (author), Eser, Bekir Engin (author), Kara, Selin (author)Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the...journal article 2022
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Reeve, Holly A. (author), Nicholson, Jake (author), Altaf, Farieha (author), Lonsdale, Thomas H. (author), Preissler, Janina (author), Lauterbach, Lars (author), Lenz, Oliver (author), Leimkühler, Silke (author), Hollmann, F. (author), Paul, C.E. (author), Vincent, Kylie A. (author)We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50%...journal article 2022
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Yun, Chul Ho (author), Kim, Jinhyun (author), Hollmann, F. (author), Park, Chan Beum (author)Enzymes are the catalyst of choice for highly selective reactions, offering nature-inspired approaches for sustainable chemical synthesis. Oxidative enzymes (e.g., monooxygenases, peroxygenases, oxidases, or dehydrogenases) catalyze a variety of enantioselective oxyfunctionalization and dehydrogenation reactions under mild conditions. To...review 2022
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Chen, Shaohang (author), Zhang, Jiaan (author), Zeng, Zhigang (author), Dai, Zongjie (author), Wang, Qinhong (author), Wever, Ron (author), Hollmann, F. (author), Zhang, W. (author)A chemoenzymatic method for the synthesis of haloethers is presented. A combination of enzymatic hypohalite synthesis with spontaneous oxidation of alkenes and nucleophilic attack by various alcohols enabled the synthesis of a wide range of haloethers. The reaction system has been characterised and current imitations have been worked out. In...journal article 2022
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Zippilli, C. (author), Bartolome, Miguel Jimenez (author), Hilberath, T. (author), Botta, Lorenzo (author), Hollmann, F. (author), Saladino, Raffaele (author)A photochemoenzymatic halodecarboxylation of ferulic acid was achieved using vanadate-dependent chloroperoxidase as (bio)catalyst and oxygen and organic solvent as sole stoichiometric reagents in a biphasic system. Performance and selectivity were improved through a phase transfer catalyst, reaching a turnover number of 660.000 for the enzyme...journal article 2022
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Hanefeld, U. (author), Hollmann, F. (author), Paul, C.E. (author)Biocatalysis has an enormous impact on chemical synthesis. The waves in which biocatalysis has developed, and in doing so changed our perception of what organic chemistry is, were reviewed 20 and 10 years ago. Here we review the consequences of these waves of development. Nowadays, hydrolases are widely used on an industrial scale for the...review 2022
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Xu, X. (author), Brasselet, H.L.Y. (author), Jongkind, E.P.J. (author), Alcalde, Miguel (author), Paul, C.E. (author), Hollmann, F. (author)In this study, we developed a new bienzymatic reaction to produce enantioenriched phenylethanols. In a first step, the recombinant, unspecific peroxygenase from Agrocybe aegerita (rAaeUPO) was used to oxidise ethylbenzene and its derivatives to the corresponding ketones (prochiral intermediates) followed by enantioselective reduction into the...journal article 2022
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Naapuri, Janne M. (author), Wagner, Philip K. (author), Hollmann, F. (author), Deska, Jan (author)Vanadate-dependent chloroperoxidase from Curvularia inaequalis catalyzes 5-endo-trig bromocyclizations of α-allenols to produce valuable halofunctionalized furans as versatile synthetic building blocks. In contrast to other haloperoxidases, also the more challenging 5-exo-trig halocyclizations of γ-allenols succeed with this system even...journal article 2022
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Bouchaut, B.F.H.J. (author), Hollmann, F. (author), Asveld, L. (author)The increasing societal demand for safer, biobased products, and processes<br/>creates opportunities for industrial biotechnology and chemistry. To succeed,<br/>controlled learning about new emerging risks is crucial but both fields endure<br/>difficulty in doing so by their respective regulation and risk management culture.journal article 2022
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Beltrán-Nogal, Alejandro (author), Sánchez-Moreno, Israel (author), Méndez-Sánchez, Daniel (author), Gómez de Santos, Patricia (author), Hollmann, F. (author), Alcalde, Miguel (author)The selective insertion of oxygen into non-activated organic molecules has to date been considered of utmost importance to synthesize existing and next generation industrial chemicals or pharmaceuticals. In this respect, the minimal requirements and high activity of fungal unspecific peroxygenases (UPOs) situate them as the jewel in the crown...review 2022
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Hwang, Se Yeun (author), Song, Dayoon (author), Seo, Eun Ji (author), Hollmann, F. (author), You, Youngmin (author), Park, Jin Byung (author)Photobiocatalysis is a growing field of biocatalysis. Especially light-driven enzyme catalysis has contributed significantly to expanding the scope of synthetic organic chemistry. However, photoenzymes usually utilise a rather narrow wavelength range of visible (sun)light. Triplet–triplet annihilation-based upconversion (TTA-UC) of long...journal article 2022
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Ma, Yunjian (author), Liang, Hongjing (author), Zhao, Zexin (author), Wu, Bin (author), Lan, Dongming (author), Hollmann, F. (author), Wang, Yonghua (author)Unspecific peroxygenases (UPOs, EC 1.11.2.1) are promising oxyfunctionalization catalysts because of their unique stereoselectivity. However, so far only a few UPOs have been reported. In this study, gene mining was used to identify a gene from Galerina marginata that coded for a novel UPO (GmaUPO). GmaUPO was expressed in Pichia pastoris X...journal article 2022
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Luan, Pengqian (author), Liu, Yunting (author), Li, Yongxing (author), Chen, Ran (author), Huang, Chen (author), Gao, Jing (author), Hollmann, F. (author), Jiang, Yanjun (author)An aqueous chemoenzymatic cascade reaction combining Pd-catalyzed C-C formation and enzymatic C=C asymmetric hydrogenation (AH) was developed for enantioselective synthesis of tertiary α-aryl cycloketones in good yields and excellent enantioselectivities. The stereopreference of the enzyme in AH of α-aryl cyclohexenones was studied. An...journal article 2021
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Alagumalai, Avinash (author), Mahian, Omid (author), Hollmann, F. (author), Zhang, Wuyuan (author)Versatile bio-derived catalysts have been under dynamic investigation as potential substitutes to conventional chemical catalysts for sustainable biodiesel production. This is because of their unique, low-cost benefits and production processes that are environmentally and economically acceptable. This critical review aspires to present a...review 2021
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Lin, Richen (author), Deng, Chen (author), Zhang, Wuyuan (author), Hollmann, F. (author), Murphy, Jerry D. (author)Bioelectrochemical technologies such as electro-fermentation and microbial CO<sub>2</sub> electrosynthesis are emerging interdisciplinary technologies that can produce renewable fuels and chemicals (such as carboxylic acids). The benefits of electrically driven bioprocesses include improved production rate, selectivity, and carbon conversion...review 2021