Title
Exploring a new dimension: Single-molecule interaction studies in sequence space
Author
Bastiaanssen, C.K.J.M.L. (TU Delft BN/Chirlmin Joo Lab) 
Contributor
Joo, C. (promotor)
Depken, S.M. (copromotor)
Degree granting institution
Delft University of Technology
Date
2024-07-10
Abstract
Interactions are essential to life. At the cellular level, the most fundamental processes rely on interactions between DNA, RNA, and proteins. Given that the structure and function of these molecules are determined by their sequence, it is ultimately the sequence that dictates the strength and specificity of these interactions. Thus, to improve our understanding of biological processes, and even predict or manipulate them, it is imperative to obtain a comprehensive and quantitative overview of the relationship between sequence and interactions. One valuable method for studying these interactions in detail is single-molecule fluorescence microscopy. However, this technique is expensive and labor-intensive, making it impractical for large sequence libraries. In this thesis, we introduce a novel approach called SPARXS: Single-molecule Parallel Analysis for Rapid eXploration of Sequence space. By integrating single-molecule fluorescence microscopy with next-generation sequencing, this platform enables the simultaneous profiling of interactions with thousands of different DNA or RNA sequences at the single-molecule level.
Subject
Argonaute
Biophysics
DNA hybridization
FRET
High-throughput
Interactions
Protein-nucleic acid interactions
Sequence
Single-molecule fluorescence microscopy
SPARXS
To reference this document use:
https://doi.org/10.4233/uuid:ce292f66-d76f-4245-83b9-878e136673ff
ISBN
978-94-6384-595-3
Embargo date
2024-07-15
Part of collection
Institutional Repository
Document type
doctoral thesis
Rights
© 2024 C.K.J.M.L. Bastiaanssen