Print Email Facebook Twitter In Vivo Prevention of Implant-Associated Infections Caused by Antibiotic-Resistant Bacteria through Biofunctionalization of Additively Manufactured Porous Titanium Title In Vivo Prevention of Implant-Associated Infections Caused by Antibiotic-Resistant Bacteria through Biofunctionalization of Additively Manufactured Porous Titanium Author van Hengel, I.A.J. (TU Delft Biomaterials & Tissue Biomechanics) van Dijk, B. (University Medical Center Utrecht) Modaresifar, K. (TU Delft Biomaterials & Tissue Biomechanics) Hooning van Duyvenbode, J. Fred F. (University Medical Center Utrecht) Nurmohamed, F. Ruben H.A. (University Medical Center Utrecht) Leeflang, M.A. (TU Delft Biomaterials & Tissue Biomechanics) Fluit, A.C. (University Medical Center Utrecht) Fratila-Apachitei, E.L. (TU Delft Biomaterials & Tissue Biomechanics) Apachitei, I. (TU Delft Biomaterials & Tissue Biomechanics) Weinans, Harrie (TU Delft Biomaterials & Tissue Biomechanics; University Medical Center Utrecht) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) Date 2023 Abstract Additively manufactured (AM) porous titanium implants may have an increased risk of implant-associated infection (IAI) due to their huge internal surfaces. However, the same surface, when biofunctionalized, can be used to prevent IAI. Here, we used a rat implant infection model to evaluate the biocompatibility and infection prevention performance of AM porous titanium against bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). The specimens were biofunctionalized with Ag nanoparticles (NPs) using plasma electrolytic oxidation (PEO). Infection was initiated using either intramedullary injection in vivo or with in vitro inoculation of the implant prior to implantation. Nontreated (NT) implants were compared with PEO-treated implants with Ag NPs (PT-Ag), without Ag NPs (PT) and infection without an implant. After 7 days, the bacterial load and bone morphological changes were evaluated. When infection was initiated through in vivo injection, the presence of the implant did not enhance the infection, indicating that this technique may not assess the prevention but rather the treatment of IAIs. Following in vitro inoculation, the bacterial load on the implant and in the peri-implant bony tissue was reduced by over 90% for the PT-Ag implants compared to the PT and NT implants. All infected groups had enhanced osteomyelitis scores compared to the noninfected controls. Subject additive manufacturingtitanium bone implantssurface biofunctionalizationMRSAimplant-associated infectionbone infection model To reference this document use: http://resolver.tudelft.nl/uuid:dad352b0-ca02-4711-b2e2-53b66b20fe2f DOI https://doi.org/10.3390/jfb14100520 ISSN 2079-4983 Source Journal of Functional Biomaterials, 14 (10) Part of collection Institutional Repository Document type journal article Rights © 2023 I.A.J. van Hengel, B. van Dijk, K. Modaresifar, J. Fred F. Hooning van Duyvenbode, F. Ruben H.A. Nurmohamed, M.A. Leeflang, A.C. Fluit, E.L. Fratila-Apachitei, I. Apachitei, Harrie Weinans, A.A. Zadpoor Files PDF jfb_14_00520.pdf 2.5 MB Close viewer /islandora/object/uuid:dad352b0-ca02-4711-b2e2-53b66b20fe2f/datastream/OBJ/view