Print Email Facebook Twitter Polyhydroxyalkanoate production in a biofilm by mixed culture phototrophic bacteria Title Polyhydroxyalkanoate production in a biofilm by mixed culture phototrophic bacteria Author Hülsen, Tim (University of Queensland) Venturato, Daniel (University of Queensland; Urban Utilities, Brisbane) Chan, Clement (University of Queensland) Vandi, Luigi (University of Queensland) Laycock, Bronwyn (University of Queensland) Pratt, Steven (University of Queensland) Stegman, Samuel (University of Queensland) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology) Batstone, Damien J. (University of Queensland) Date 2024 Abstract Anoxygenic purple phototrophic bacteria (PPB) were utilised in an 80 L biofilm flat plate photobioreactor to generate polyhydroxyalkanoate (PHA) over 44 cycles, with acetate as feed. Over the cycles, net PHA yield (growth + accumulation) averaged 21% while accumulation yield averaged 55%. Average PHA content was 35 wt% volatile solids (VS), with the majority (>80%) being harvested from the biofilm at 100 gTotal solids (TS) L−1. The PPB microbial population averaged 45% of total population. Detailed cycle studies indicated that PHA content (and yield) peaked at 0.5–1 d into the accumulation stage (peak of 53 wt% VS), suggesting that cycle time optimisation could improve both yield and selection of PHA accumulators. The resulting polymeric material was comprised of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with 95.5 mol% 3-hydroxybutyrate and 4.5 mol% 3-hydroxyvalerate content, but the molecular weight, at >1090 kDa, is unusually high for extracted, microbially produced PHA and a feature of this work. Subject BiofilmPolyhydroxyalkanoatesPurple phototrophic bacteriaResource recovery To reference this document use: http://resolver.tudelft.nl/uuid:ba0940e6-e49a-4bf2-9b94-e5d174ac651f DOI https://doi.org/10.1016/j.jclepro.2023.140001 ISSN 0959-6526 Source Journal of Cleaner Production, 434 Part of collection Institutional Repository Document type journal article Rights © 2024 Tim Hülsen, Daniel Venturato, Clement Chan, Luigi Vandi, Bronwyn Laycock, Steven Pratt, Samuel Stegman, Mark C.M. van Loosdrecht, Damien J. Batstone Files PDF 1_s2.0_S0959652623041598_main.pdf 3.67 MB Close viewer /islandora/object/uuid:ba0940e6-e49a-4bf2-9b94-e5d174ac651f/datastream/OBJ/view