Print Email Facebook Twitter Incorporating strontium enriched amorphous calcium phosphate granules in collagen/collagen-magnesium-hydroxyapatite osteochondral scaffolds improves subchondral bone repair Title Incorporating strontium enriched amorphous calcium phosphate granules in collagen/collagen-magnesium-hydroxyapatite osteochondral scaffolds improves subchondral bone repair Author Xu, J. (Erasmus MC) Vecstaudza, Jana (Riga Technical University) Wesdorp, Marinus A. (Erasmus MC) Labberté, Margot (University College Dublin) Salerno, Manuela (Rizzoli Orthopaedic Institute) Kok, Joeri (Eindhoven University of Technology) van Rietbergen, Bert (Eindhoven University of Technology) van Osch, G.J.V.M. (TU Delft Biomaterials & Tissue Biomechanics; Erasmus MC) Locs, Janis (Riga Technical University) Brama, Pieter A.J. (University College Dublin) Date 2024 Abstract Osteochondral defect repair with a collagen/collagen-magnesium-hydroxyapatite (Col/Col-Mg-HAp) scaffold has demonstrated good clinical results. However, subchondral bone repair remained suboptimal, potentially leading to damage to the regenerated overlying neocartilage. This study aimed to improve the bone repair potential of this scaffold by incorporating newly developed strontium (Sr) ion enriched amorphous calcium phosphate (Sr-ACP) granules (100–150 μm). Sr concentration of Sr-ACP was determined with ICP-MS at 2.49 ± 0.04 wt%. Then 30 wt% ACP or Sr-ACP granules were integrated into the scaffold prototypes. The ACP or Sr-ACP granules were well embedded and distributed in the collagen matrix demonstrated by micro-CT and scanning electron microscopy/energy dispersive x-ray spectrometry. Good cytocompatibility of ACP/Sr-ACP granules and ACP/Sr-ACP enriched scaffolds was confirmed with in vitro cytotoxicity assays. An overall promising early tissue response and good biocompatibility of ACP and Sr-ACP enriched scaffolds were demonstrated in a subcutaneous mouse model. In a goat osteochondral defect model, significantly more bone was observed at 6 months with the treatment of Sr-ACP enriched scaffolds compared to scaffold-only, in particular in the weight-bearing femoral condyle subchondral bone defect. Overall, the incorporation of osteogenic Sr-ACP granules in Col/Col-Mg-HAp scaffolds showed to be a feasible and promising strategy to improve subchondral bone repair. Subject Amorphous calcium phosphateOsteochondral defectRegenerative medicineStrontiumTissue engineering To reference this document use: http://resolver.tudelft.nl/uuid:6f94c28c-fbae-458e-a757-6331d1561b25 DOI https://doi.org/10.1016/j.mtbio.2024.100959 ISSN 2590-0064 Source Materials Today Bio, 25 Part of collection Institutional Repository Document type journal article Rights © 2024 J. Xu, Jana Vecstaudza, Marinus A. Wesdorp, Margot Labberté, Manuela Salerno, Joeri Kok, Bert van Rietbergen, G.J.V.M. van Osch, Janis Locs, Pieter A.J. Brama, More Authors Files PDF 1-s2.0-S2590006424000188-main.pdf 12.22 MB Close viewer /islandora/object/uuid:6f94c28c-fbae-458e-a757-6331d1561b25/datastream/OBJ/view