Print Email Facebook Twitter Enzyme engineering for biocatalysis Title Enzyme engineering for biocatalysis Author Paul, C.E. (TU Delft BT/Biocatalysis) Hanefeld, U. (TU Delft BT/Biocatalysis) Hollmann, F. (TU Delft BT/Biocatalysis) Qu, Ge (Chinese Academy of Sciences) Yuan, Bo (Chinese Academy of Sciences) Sun, Zhoutong (Chinese Academy of Sciences) Date 2024 Abstract Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes frequently lack the requisite attributes for effective organic synthesis. The inherent limitations in stability, catalytic activity, and selectivity of wild-type enzymes often hinder their suitability for chemical synthesis. Over the past 25 years, there has been an unprecedented advancement in protein engineering tools, empowering enzymologists to customise enzymes to precisely meet the demands of organic synthesis. In this discussion, we delineate some of the most crucial techniques in enzyme engineering and their significance in facilitating chemical synthesis. Subject BiocatalysisDirected evolutionIterative saturation mutagenesisSemi-rational design To reference this document use: http://resolver.tudelft.nl/uuid:3d0c1cf8-8e92-459a-8f6c-01f204cb1cdf DOI https://doi.org/10.1016/j.mcat.2024.113874 ISSN 2468-8231 Source Molecular Catalysis, 555 Part of collection Institutional Repository Document type journal article Rights © 2024 C.E. Paul, U. Hanefeld, F. Hollmann, Ge Qu, Bo Yuan, Zhoutong Sun Files PDF 1-s2.0-S2468823124000579-main.pdf 3.87 MB Close viewer /islandora/object/uuid:3d0c1cf8-8e92-459a-8f6c-01f204cb1cdf/datastream/OBJ/view