Print Email Facebook Twitter Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair Title Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair: In Vitro, Ex Vivo and In Vivo Evaluation Author Xu, J. (Erasmus MC) Fahmy-Garcia, Shorouk (Erasmus MC) Wesdorp, Marinus A. (Erasmus MC) Forte, Lucia (Fin-Ceramica Faenza) De Luca, Claudio (Fin-Ceramica Faenza) Filardo, Giuseppe (Rizzoli Orthopaedic Institute) Labberté, Margot (University College Dublin) Kok, Joeri (Eindhoven University of Technology) Nickel, Joachim (Universitätsklinikum Würzburg) van Osch, G.J.V.M. (TU Delft Biomaterials & Tissue Biomechanics; Erasmus MC) Date 2023 Abstract Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet-derived growth factor-BB (PDGF-BB) onto said scaffold. The in vitro release kinetics of BMP-2/PDGF-BB demonstrated that PDGF-BB was burst released from the collagen-only layer, whereas BMP-2 was largely retained in both layers. Cell ingrowth was enhanced by BMP-2/PDFG-BB in a bovine osteochondral defect ex vivo model. In an in vivo semi-orthotopic athymic mouse model, adding BMP-2 or PDGF-BB increased tissue repair after four weeks. After eight weeks, most defects were filled with bone tissue. To further investigate the promising effect of BMP-2, a caprine bilateral stifle osteochondral defect model was used where defects were created in weight-bearing femoral condyle and non-weight-bearing trochlear groove locations. After six months, the adsorption of BMP-2 resulted in significantly less bone repair compared with scaffold-only in the femoral condyle defects and a trend to more bone repair in the trochlear groove. Overall, the adsorption of BMP-2 onto a Col/Col-Mg-HAp scaffold reduced bone formation in weight-bearing osteochondral defects, but not in non-weight-bearing osteochondral defects. Subject animal modelbiocompatible materialsbone morphogenetic proteinsosteochondral lesionplatelet-derived growth factorregenerative medicinetissue engineeringweight-bearing To reference this document use: http://resolver.tudelft.nl/uuid:2fd60cc5-4aca-4339-b51a-e11b227d133d DOI https://doi.org/10.3390/jfb14020111 ISSN 2079-4983 Source Journal of Functional Biomaterials, 14 (2) Part of collection Institutional Repository Document type journal article Rights © 2023 J. Xu, Shorouk Fahmy-Garcia, Marinus A. Wesdorp, Lucia Forte, Claudio De Luca, Giuseppe Filardo, Margot Labberté, Joeri Kok, Joachim Nickel, G.J.V.M. van Osch, More Authors Files PDF jfb_14_00111.pdf 10.12 MB Close viewer /islandora/object/uuid:2fd60cc5-4aca-4339-b51a-e11b227d133d/datastream/OBJ/view